Information om | Engelska ordet STREPTAVIDIN

Exempel på hur man kan använda STREPTAVIDIN i en mening

• Biotin binds to streptavidin and avidin with an extremely high affinity, fast on-rate, and high specificity, and these interactions are exploited in many areas of biotechnology to isolate biotinylated molecules of interest.
• The principle of pyrosequencing was first described in 1993 by, Bertil Pettersson, Mathias Uhlen and Pål Nyren by combining the solid phase sequencing method using streptavidin coated magnetic beads with recombinant DNA polymerase lacking 3´to 5´exonuclease activity (proof-reading) and luminescence detection using the firefly luciferase enzyme.
• At first, an antibody-drug conjugate is administered, which consists of a monoclonal antibody designed to target the tumour, and a chemical marker which in the case of DOTA-biotin therapy is one of the proteins avidin and streptavidin.
• Biotin-binding affinity can be impaired by chemical labeling of streptavidin, such as with amine-reactive fluorophores; flavidin is a streptavidin mutant without lysine side-chains, which retains good biotin binding characteristics after such fluorescent dye labeling where the dye couples to the amino terminus.
• One other method uses complexes of injected biocytin with avidin or streptavidin, biotin, and then peroxidase.
• lectins, streptavidin, monoclonal antibodies, phospholipase A2 and acetylcholinesterase, tailoring of bioreactive surfaces; mixed protein multilayers.
• The beads are coated with primary antibodies, specific-specific antibodies, lectins, enzymes, or streptavidin; the linkage between magnetized beads coated materials are cleavable DNA linker cell separation from the beads when the culturing of cells is more desirable.
• When using a biotin label, streptavidin conjugated to an enzyme such as horseradish peroxidase is used to detect the DNA fragment.
• In the second step, the amplified product is attached to a streptavidin column and washed with NaOH to remove the unbiotinylated strand.
• Fluorophore tagged streptavidin is added to the bioengineered MHC monomers, and the biotin-streptavidin interaction causes four MHC monomers to bind to the streptavidin and create a tetramer.
• In biochemical applications, streptavidin, which also binds very tightly to biotin, may be used interchangeably with NeutrAvidin.
• The ZFPs are applied to microtitre wells coated with streptavidin and a biotinylated target oligonucleotide.
• In 2013, a group at the Beatson West of Scotland Cancer Centre reported an antibody-free protein visualization standard for immunoblotting based on detection of MCCC1 using streptavidin conjugates.
• In addition, Ting and her lab developed monovalent streptavidin, site-specific biotinylation in mammalian cells, small monovalent quantum dots for single molecule imaging, APEX2 as a genetic tag for electron microscopy (analogous to green fluorescent protein but visible by electron microscopy), split horseradish peroxidase for visualization of synapses in vivo, FLARE (fast light- and activity-regulated expression) for gaining genetic access to activated neural ensembles, SPARK (specific protein association tool giving transcriptional readout with rapid kinetics) for transcriptional readout of protein-protein interactions, and PRIME (probe incorporation mediated by enzymes) – a protein labeling technique that enables scientists to capitalize on the brightness, photostability, small size, and chemical diversity of small-molecule probes as an alternative to green fluorescent protein.
• The initial procedure to isolate RAD tags involved digesting DNA with a particular restriction enzyme, ligating biotinylated adapters to the overhangs, randomly shearing the DNA into fragments much smaller than the average distance between restriction sites, and isolating the biotinylated fragments using streptavidin beads.
• MHC tetramers consist of four MHC molecules, a tetramerization agent and a fluorescently labeled protein (usually streptavidin).
• DNA is then extracted from the cells, sonicated and enriched for regions containing the biotinylated molecule of interest by incubation with streptavidin magnetic beads, which have a very high affinity for biotin.
• Microbeads are pre-coupled with a ligand; a biomolecule such as antibody, streptavidin, protein, antigen, DNA/RNA, or other molecule.
• If streptavidin-ALP (streptavidin and alkaline phosphatase conjugate) is used, then using BCIP/NBT-plus (a mixture of 5-bromo-4-chloro-3-indolyl phosphate and nitroblue tetrazolium chloride) as a substrate will produce more distinct spots that are easier to analyze.
• In contrast to chromatin immunoprecipitation (ChIP)-based methods of identifying DNA double-strand breaks (DSBs) by labeling DNA repair proteins, BLESS utilizes biotinylated DNA linkers to directly label genomic DNA in situ which allows for high-specificity enrichment of samples on streptavidin beads and the subsequent sequencing-based DSB mapping to nucleotide resolution.

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